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. 2021 May 24;3:669763. doi: 10.3389/fmedt.2021.669763

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Copyright © 2021 Molino, Fukuda, Molino and Wallace.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(i) Surface profile of the biosynthetic cell culture platform. Scanning electron microscopy (SEM) images of (a) a 75:25 PLA:PLGA fibre, and (b,c) hybrid platforms made from 75:25 PLA:PLGA fibres and PPy doped with pTS (b) and HA (c). The broken line separates the area on the PPy substrate where the fibres have been removed (pointed by the arrows). Also shown are profilometry images of the hybrid platform in the (d) presence and (e) absence of PLA:PLGA fibres, and (f) height profile obtained from image (e). (ii) Fluorescence images of differentiated, multinucleated desmin (green) expressing myotubes on PPy/pTS substrate (a,b) with and (c,d) without the presence of PLA:PLGA fibre array. Cell nuclei are shown in blue. Scale bars are 200 mm. Reproduced with permission from Razal et al. (168).