Table 3.
Various conditions used in various studies with ES of PC12 cells.
| Polymer | Stimulus apparatus | ES | Scaffold |
|---|---|---|---|
| PANI on ITO (106) | The ITO covered with a PANI film was set at the bottom of the cell culture plate, and two wires were set in contact with the well for the electric stimuli | 100-μA amplitude, 0.8-ms pulse width, and 1-s repeat interval stimulating the biphasic rectangular current pulse for 1, 2, and 4 h | Film |
| PCLF-PPy (107) | The membrane was placed at the bottom of the well, and a silicon tube with two platinum wires was placed in the well to make contact with the membrane scaffold without exposing the wires to the culture medium | 10 μA or 20 Hz for 1 h/d for 2 days | Membrane |
| PEDOT:PSS (108) | The opposite ends of the patterned nanoparticles arrays were painted with silver epoxy electrodes for the electric stimuli | Monophasic pulsed current at 250 Hz with a 2-ms pulse width and an amplitude of 1 mA for 2 h | Patterned multifunctional PEDOT:PSS nanoparticle arrays |
| PEO/PEDOT:PSS (86) | The cell chamber was made over an ITO electrode coated with the material, and the electrical stimuli were applied to the ITO | Biphasic square wave (100 mV cm−1 electric field; duration of 100 ms; interval of 100 ms) in 1 h/d for 5 days | Fibres |
| PLAAP (109) | The membrane was placed at the bottom of the cell plate, and two platinum electrodes were placed in contact with the lengthwise boundaries of the membrane | 1 Hz, 0.1 V in 1 h/d for 4 days | Film |
| PPy/PDLLA (110) | The conduits were placed between a PDMS film and a PMDS well-attached to two silver wire electrodes | 100 mV for 2 h | Conduit |
| PPy (24) | PPy acted as the anode, and lengthways to the well a gold wire acted as the cathode; the system also had a silver wire as a quasi-reference electrode | 100 mV for 2 h | Film over ITO |
| PPy-coated PLGA (111) | The fibres were placed between a PDMS film and a PMDS well, which were attached to two silver wire electrodes | 100 mV/cm or 10 mV/cm for 2 h | Fibres |
| PPy-CS and PPy-CS-Col (112) | The material was set at the bottom of the cubic chamber, and on two opposite sides of the well were PVDF sheets to link the media to the other two wells, whichwere connected to stainless steel meshes for the power supplier | ±1-m A pulse duration of 0.1 ms, with a steady state interval of 3.8 ms for 144 h | Film |
| PPy-NGF (113) | PPy acted as the anode, and lengthways to the well a gold wire acted as the cathode; the system also had a silver wire as a quasi-reference electrode | 100 mV for 2 h | Films |
| PPy-PLLA (114) | The fibres were placed between a PDMS film and a PMDS well-attached to two gold wire electrodes | 100, 200, 400, and 800 mV/cm for 4 h | Fibres |
| PS/PANI (115) | The fibres were set at the bottom of the well with one end of the mesh connected with a platinum and the other with a silver electrode | 100 mV/cm in 1 h/d for 5 days | Fibres |