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. 2022 Jan 13;17(1):e0261014. doi: 10.1371/journal.pone.0261014

Fig 1. Study rationale and assay design.

Fig 1

Panel A illustrates the study’s rationale and sketches the layout of the ARTIC and MRL primer pools across the SARS-CoV-2 genome. Small and long arrows indicate short and long amplicons, respectively. Red arrows indicate a mutation with potential to alter the primer binding site. Panel B shows the design of the three assays developed and assessed in the present study. Assay-1 is based on short-amplicons generated with the ARTIC primer pool. Assay-2 is based on long-amplicons made with MRL primers followed by short-read sequencing on MiSeqDx. Assay-3 is based on long-amplicons sequenced by long read sequencing technology on MinION platform.