Table 1. Primer sequences for real-time quantitative polymerase chain reaction.
| Primer | Sequence | Product size | GenBank accession number |
|---|---|---|---|
| MyoD 1 | 5’-GAC GGC ATG ATG GAG TAC AG-3’ (forward) | 201 bp5 | AY641567.1 |
| 5’-AGC TTC AGC TGG AGG CAG TA-3’ (reverse) | |||
| MyoG 2 | 5’-CCT TTC CCA CTC CTC TCC AAA-3’ (forward) | 175 bp | AY560111.3 |
| 5’-GAC CTT GGT CGA AGA GCA ACT-3’ (reverse) | |||
| mTOR 3 | 5’-GGG TGG AGG TGA ACT TAC AGG-3’ (forward) | 185 bp | XM_010723001.3 |
| 5’-TCC TGG CTC ATT TCA CGG AG-3’ (reverse) | |||
| GAPDH 4 | 5’-GAG GGT AGT GAA GGC TGC TG-3’ (forward) | 200 bp | U94327.1 |
| 5’-CCA CAA CAC GGT TGC TGT AT-3’ (reverse) |
1MYOD, Myogenic Determination Factor 1
2MYOG, Myogenin
3mTOR, Mechanistic Target of Rapamycin
4GAPDG, Glyceraldehyde-3-phosphate dehydrogenase
5bp, number of base pairs