Table 2. Effect of heat stress (43°C) and knockdown of mTOR on RBC2 and NC line SC proliferation¹.

Line	Temperature2	Knockdown3	Sampling time				P-value4
			0 h	24 h	48 h	72 h
RBC2	38	Control	0.14a,y ± 0.01	0.19cd,y ± 0.03	0.31de,y ± 0.10	0.62d,z ± 0.08	< 0.0001
		mTOR	0.13a,y ± 0.00	0.13d,y ± 0.03	0.17e,y ± 0.09	0.31e,z ± 0.08	< 0.0001
	43	Control	0.13a,x ± 0.01	0.20bcd,x ± 0.04	0.51cd,y ± 0.10	1.57b,z ± 0.08	< 0.0001
		mTOR	0.13a,x ± 0.00	0.13d,x ± 0.03	0.24de,y ± 0.10	0.71d,z ± 0.08	< 0.0001
NC	38	Control	0.13a,x ± 0.01	0.26bc,x ± 0.03	0.63bc,y ± 0.10	1.46bc,z ± 0.08	< 0.0001
		mTOR	0.13a,x ± 0.00	0.18cd,x ± 0.03	0.38cde,y ± 0.10	0.66d,z ± 0.09	< 0.0001
	43	Control	0.14a,w ± 0.01	0.46a,x ± 0.03	1.43a,y ± 0.10	2.67a,z ± 0.10	< 0.0001
		mTOR	0.14a,x ± 0.01	0.29b,x ± 0.03	0.80b,y ± 0.10	1.30c,z ± 0.11	< 0.0001
P-value5		L	0.8123	<0.0001	<0.0001	<0.0001
		T	0.5876	0.0008	<0.0001	<0.0001
		K	0.4948	0.0002	<0.0001	<0.0001
		L × T × K	0.7781	0.2904	0.3800	0.9826

¹ Each value represents mean DNA concentration (μg / well) ± Standard error of mean (SEM)

² Incubation temperature (°C) during proliferation and differentiation

³ Control = transfecting cells with a negative control sequence; mTOR = knockdown of mTOR

⁴ Effect of sampling times within each cell line, temperature, and knockdown of mTOR

⁵ Effect of line (L), temperature (T), and knockdown of mTOR (K), and the interaction effect among line, temperature, and knockdown of mTOR (L × T × K) within each sampling time

^a-f Mean DNA concentration (μg / well ± SEM) within a column (sampling time) without a common letter are significantly different

^w-z Mean DNA concentration (μg / well ± SEM) within a row (temperature and cell line) without a common letter are significantly different

P ≤ 0.05 was considered as significant different