Table 5. Effect of heat stress (43°C) and knockdown of mTOR at the beginning of differentiation on differentiation of RBC2 and NC line SCs¹.

Line	Temperature2	Knockdown3	Sampling time				P-value4
			0 h	24 h	48 h	72 h
RBC2	38	Control	6.93c,w ± 0.36	15.79f,x ± 0.43	40.45f,y ± 1.08	51.77g,z ± 0.59	< 0.0001
		mTOR	6.81c,w ± 0.32	15.46f,x ± 0.43	30.05g,y ± 1.08	38.37h,z ± 0.77	< 0.0001
	43	Control	12.31b,w ± 0.32	51.54d,x ± 0.47	72.35d,z ± 0.97	68.15e,y ± 0.54	< 0.0001
		mTOR	11.63b,w ± 0.30	48.93e,x ± 0.61	62.08e,z ± 0.88	56.17f,y ± 0.59	< 0.0001
NC	38	Control	12.36b,w ± 0.36	67.98b,x ± 0.61	111.20b,z ± 1.08	83.60b,y ± 0.59	< 0.0001
		mTOR	12.38b,w ± 0.31	65.35c,x ± 0.61	101.31c,z ± 1.25	80.67c,y ± 0.66	< 0.0001
	43	Control	24.75a,w ± 0.36	75.19a,x ± 0.47	118.00a,z ± 2.20	99.95a,y ± 0.66	< 0.0001
		mTOR	24.82a,w ± 0.36	69.43b,x ± 0.47	104.92c,z ± 1.32	77.61d,y ± 0.66	< 0.0001
P-value5		L	< 0.0001	< 0.0001	< 0.0001	< 0.0001
		T	< 0.0001	< 0.0001	< 0.0001	< 0.0001
		K	0.4613	< 0.0001	< 0.0001	< 0.0001
		L × T × K	0.5239	0.5720	0.3674	< 0.0001

¹ Each value represents mean creatine kinase activity (Unit / well) ± Standard error of mean (SEM)

² Incubation temperature (°C) during proliferation and differentiation

³ Control = transfecting cells with a negative control sequence; mTOR = knockdown of mTOR

⁴ Effect of sampling times within each cell line, temperature, and knockdown of mTOR

⁵ Effect of line (L), temperature (T), and knockdown of mTOR (K), and the interaction effect among line, temperature, and knockdown of mTOR (L × T × K) within each sampling time

^a-f Mean creatine kinase activity (Unit / well ± SEM) within a column (sampling time) without a common letter are significantly different

^w-z Mean creatine kinase activity (Unit / well ± SEM) within a row (temperature and cell line) without a common letter are significantly different

P ≤ 0.05 was considered as significant different