Table 1. Primer pairs for quantitative RT-PCR analysis of target rabbit genes.
Target gene | Forward (F) and reverse (R) primer sequence | Product length | NCBI accession |
---|---|---|---|
CD31 | F: GCATTGGCAAGGTGGTGAAG | 130 | XM_008271715.2 |
XM_008271716.2 | |||
R: TGGCAGCTCACTTCTATGGC | |||
CD34 | F: TCCCGGAAGACCTTGATTGC | 128 | XM_008268471.2 |
R: TAAGGGTCTTCGCCCAGCC | |||
CD45 | F: AAGACAACGGTGCAGGAAGG | 134 | XM_008268693.2 |
R: CCCGGCAACAAACACTTCTG | |||
vWF | F: TGAAGCTCCAGCGCATTGAA | 146 | NM_001329088.1 |
R: ATCTGGCAAGGTCCACACAT | |||
VEGFA | F: AGTTCGAGGAAAGGGCAAGG | 157 | XM_017345155.1 |
R: ACGCGAGTCTGTGTTTTTGC | |||
VEGFR1 | F: GTGCACGGTCAACAAGTTCC | 80 | XM_008275216.2 |
R: TGTGGCGCATTGTTCTGTTG | |||
VEGFR2 | F: GGTGCTTCTCCGTATCCTGG | 222 | NM_001195670.1 |
R: GTAGTCTTTGCCACCCTGCT | |||
ANGPT1 | F: TGTGCCCTCATGCTTACAGG | 236 | XM_008255827.2 |
R: CTCGGTCTCCAGATCGCTTC | |||
ANGPT2 | F: TGGCTGGGAAACGAGTTTGT | 198 | XM_002720896.3 |
R: TGGTTGGCTGATGCTGCTTA | |||
Tie2 | F: CAAGAACAACCCGGATCCCA | 117 | XM_017340937.1 |
R: TCCTTCTTGATGCGTGCCTT | |||
CDH5 | F: CAGGTACGAGATCGTGGTGG | 120 | XM_008257382.2 |
R: GTCTGCGTGAAGATGGGGAA | |||
CD90 | F: CCTGAGCCTCTCAAGACAGC | 160 | XM_002722718.3 |
R: TGTGATGGCACTACACACGG | |||
CD105 | F: AGCATGATCAGCAACGAGGT | 182 | XM_008251029.2 |
R: GAGCTCACCTGCACAAAACC | |||
CD73 | F: GCAGTTGAGGGTCGGATCAA | 274 | XM_002714557.3 |
R: CTGAGTTGCTGATCCGAGTGA | |||
CD44 | F: TCAAACAGCCATGCAGCAAC | 180 | XM_008269684.2 |
R: CCACCATGGAAGCCAATCCT | |||
CD29 | F: GAATGCCAAATGGGACACGC | 111 | XM_008274741.2 |
R: AGGATTTTCACCGGCACTCA | |||
GAPDH [31] | F(PS182): TGACGACATCAAGAAGGTGGTG | 120 | NM_001082253 |
R(PS183): GAAGGTGGAGGAGTGGGTGTC |