Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | w67c23 | Bloomington Drosophila Stock Center | FBal0095147 | |
| Gene (Drosophila melanogaster) | w1118 | Bloomington Drosophila Stock Center | FBal0018186RRID:BDSC_6598 | |
| Gene (Drosophila melanogaster) | p53 (p535A1-4) | Bloomington Drosophila Stock Center | FLYB:FBgn0039044; RRID:BDSC_6815 | FBal0138188 |
| Gene (Drosophila melanogaster) | p53 (p53A2.3) | Robin et al., 2019 | FLYB:FBgn0039044 | See Materials and Methods, Section 2 |
| Gene (Drosophila melanogaster) | p53 (p53B41.5) | this study | FLYB:FBgn0039044 | See Materials and Methods, Section 2 |
| Gene (Drosophila melanogaster) | okra (okraAA) | Ghabrial et al., 1998 | FLYB:FBgn0002989 | Obtained from T. Schupbach |
| Gene (Drosophila melanogaster) | okra (okraRU) | Bloomington Drosophila Stock Center | FLYB:FBgn0002989; RRID:BDSC_5098 | FBal0013236;Obtained from T. Schupbach |
| Gene (Drosophila melanogaster) | mei-W68 (Df(2 R)BSC782) | Bloomington Drosophila Stock Center | FLYB:FBgn0002716; RRID:BDSC_27354 | |
| Gene (Drosophila melanogaster) | mei-W68 (mei-W681) | Bloomington Drosophila Stock Center | FLYB:FBgn0002716; RRID:BDSC_4932 | FBal0012191 |
| Genetic reagent (Drosophila melanogaster) | hid-GFP | Tanaka-Matakatsu et al., 2009 | FLYB:FBgn0003997; RRID:BDSC_50751 | Obtained from W. Du |
| Genetic reagent (Drosophila melanogaster) | GFP-p53A | Zhang et al., 2015 | FLYB:FBtp0111619 | |
| Genetic reagent (Drosophila melanogaster) | mCh-p53B | Zhang et al., 2015 | FLYB:FBtp0098077 | |
| Sequence-based reagent | p53 gRNA | This study | 5’:CCTGGAGCA CGGAAGATTCTTG; 3’:GATCCACAG GCGTAGCCAGGTGG |
|
| Sequence-based reagent | primer #501 | This study | PCR primer | CCAACAAGAT CGCTTGATCAGATA |
| Sequence-based reagent | primer #1,085 | This study | PCR primer | GGCCATGGG TTCCGTGGTCA |
| Sequence-based reagent | primer #1,061 | This study | PCR primer | GAGTCAGCAG TTCGGGTCTC |
| Antibody | Anti-GFP (Rabbit polyclonal) | Invitrogen | Cat# A11122 | IF(1:500) |
| Antibody | Anti-dsRed (Rabbit polyclonal) | Clontech | Cat# 632,496 | IF(1:200) |
| Antibody | Anti-dsRed (mouse polyclonal) | Clontech | Cat# 632,392 | IF(1:200) |
| Antibody | Anti-Hts 1B1 (mouse monoclonal) | Developmental Studies Hybridoma Bank | RRID:AB_528070 | IF(1:20) |
| Antibody | Anti-γH2Av (mouse monoclonal) | Developmental Studies Hybridoma Bank | RRID:AB_2618077 | IF(1:1000) |
| Antibody | Anti-orb 4H8 (mouse monoclonal) | Developmental Studies Hybridoma Bank | RRID:AB_528418 | IF(1:500) |
| Antibody | Anti-Vasa (rat monoclonal) | Developmental Studies Hybridoma Bank | RRID:AB_10571464 | IF(1:100) |
| Antibody | Alexa Fluor 488 anti-mouse (polyclonal) | Invitrogen | Cat# A11011 | IF(1:1000) |
| Antibody | Alexa Fluor 488 anti-rabbit (polyclonal) | Invitrogen | Cat# A11008 | IF(1:1000) |
| Antibody | Alexa Fluor 568 anti-mouse (polyclonal) | Invitrogen | Cat# A11004 | IF(1:1000) |
| Antibody | Alexa Fluor 568 anti-rabbit (polyclonal) | Invitrogen | Cat# A10042 | IF(1:1000) |
| Antibody | Alexa Fluor 488 anti-mouse IgG1 (polyclonal) | Invitrogen | Cat# A21121 | IF(1:1000) |
| Antibody | Alexa Fluor 568 anti-mouse IgG2b (polyclonal) | Invitrogen | Cat# A21144 | IF(1:1000) |