Key resources table.
| Reagent type (species) or resource | Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293T | Laboratory cell bank of Shenzhen PKU-HKUST Medical Center |
Initially ordered from ATCC by colleagues in HKUST |
|
| Cell line (H. sapiens) | HLEB3 | ATCC | ATCC CRL-11421, RRID:CVCL_6367 | |
| Strain, strain background (Danio rerio) | pikfyveΔ8 mutant | This paper | Maintained in Shenzhen PKU-HKUST Medical Center | |
| Strain, strain background (D. rerio) | Tg(ubi:PIKFYVEWT) | This paper | Maintained in Shenzhen PKU-HKUST Medical Center | |
| Strain, strain background (D. rerio) | Tg(ubi:PIKFYVEG1943E) | This paper | Maintained in Shenzhen PKU-HKUST Medical Center | |
| Strain, strain background (D. rerio) | Tg(cryaa:DsRed;il-1b:GFP-F) | doi.10.1242/dmm.014498 | ||
| Chemical compound, drug | YM201636 | Selleck, China | S1219 | 800 nM for 4 hr |
| Chemical compound, drug | bafilomycin A1 | MedChemExpress, NJ | HY-100558 | 1 μM for 4.5 hr |
| Antibody | Anti-PIKFYVE antibody (rabbit polyclonal) | Abcam, UK | Cat# ab137907 | WB (1:1000) |
| Antibody | Anti-GAPDH antibody (rabbit polyclonal) | Abcam, UK | Cat# ab9485, RRID:AB_307275 | WB (1:1000) |
| Antibody | Anti-Rabbit IgG H&L (goat polyclonal) | Abcam, UK | Cat# ab205718, RRID:AB_2819160 | WB (1:5000) |
| Antibody | Anti-Lens Fiber Cell Marker antibody [ZL-1] (mouse monoclonal) | Abcam, UK | Cat# ab185979 | IF (1:100) |
| Antibody | Anti-Mouse-555 secondary antibody (donkey polyclonal) | Thermo Fisher Scientific, USA | Cat# A-31570, RRID:AB_2536180 | IF (1:400) |
| Sequence-based reagent (H. sapiens) | Human PIKFYVE genotyping fwd | This paper | PCR primer | TTTTGACCTTCTCTTGATTAGAGG |
| Sequence-based reagent (H. sapiens) | Human PIKFYVE genotyping rev | This paper | PCR primer | AAATATGGCCTAGTAACCAAAGTTAAA |
| Sequence-based reagent (H. sapiens) | Human PIKFYVE cDNA cloning fwd | This paper | PCR primer | ATGGCCACAGATGATAAGAC |
| Sequence-based reagent (H. sapiens) | Human PIKFYVE cDNA cloning rev | This paper | PCR primer | TCAGCAATTCAGACCCAAGCCTG |
| Sequence-based reagent (H. sapiens) | Human PIKFYVE qPCR fwd | This paper | PCR primer | CGTCCCCAACACTGGACTCTGC |
| Sequence-based reagent (H. sapiens) | Human PIKFYVE qPCR rev | This paper | PCR primer | CCCTGGCCTCCTTCTGCTCTCTC |
| Sequence-based reagent (H. sapiens) | Human GAPDH qPCR fwd | This paper | PCR primer | CGAGATCCCTCCAAAATCAA |
| Sequence-based reagent (H. sapiens) | Human GAPDH qPCR rev | This paper | PCR primer | GTCTTCTGGGTGGCAGTGAT |
| Sequence-based reagent (D. rerio) | Zebrafish pikfyve genotyping fwd | This paper | PCR primer | GAGAACCTGCTCAAACTGGTGC |
| Sequence-based reagent (D. rerio) | Zebrafish pikfyve genotyping rev | This paper | PCR primer | AGATTTGACCACCATCTCCAGC |