| Genetic reagent(Mus. Musculus) |
C57BL/6JbomTac |
DBL |
RRID:IMSR_TAC:b6jbom
|
|
| Cell line (Homo sapiens) |
HepG2 |
ATCC |
HB-8065, RRID:CVCL_0027
|
|
| Cell line (Homo sapiens) |
Huh-7 |
Dr. Yoshiharu Matsuura; originally from Japanese Collection of Research Bioresources Cell Bank |
JCRB0403, RRID:CVCL_0336
|
|
| Antibody |
Anti-PPARα(Rabbit Polyclonal) |
abcam |
Cat# ab24509, RRID:AB_448110
|
WB(1:1000) |
| Antibody |
Anti-HSP90(Rabbit Polyclonal) |
Cell Signaling Technology |
Cat# 4,877 S, RRID:AB_2233307
|
WB(1:1000) |
| Sequence-based reagent |
MIR20B/Mir20b(MIR20B/Mir20b mimic) |
GenePharma |
N/A |
Sequence: CAAAGUGCUCAUAGUGCAGGUAG |
| Sequence-based reagent |
anti-MIR20B/Mir20b (MIR20B/Mir20b inhibitor) |
GenePharma |
N/A |
Sequence: CUACCUGCACUAUGAGCACUUUG |
| Sequence-based reagent |
PPARA siRNA |
GenePharma |
N/A |
Sequence: CGGCGAGGATAGTTCTGGAAGCTTT |
| Sequence-based reagent |
PPARA shRNA |
Sigma-Aldrich |
N/A |
Sequence: GAACAGAAACAAATGCCAGTASequence:GTAGCGTATGGAAATGGGTTT |
| Sequence-based reagent |
Primers for qPCR |
This paper |
N/A |
|
| Recombinant DNA reagent |
psiCHECK-2- PPARA-WT (plasmid) |
This paper |
N/A |
|
| Recombinant DNA reagent |
psiCHECK-2- PPARA-Mut (plasmid) |
This paper |
N/A |
|
| Recombinant DNA reagent |
pOTTC385-pAAV CMV-IE IRES EGFP-Mir20b
|
This paper |
N/A |
|
| Recombinant DNA reagent |
pOTTC385-pAAV CMV-IE IRES EGFP-anti-Mir20b
|
This paper |
N/A |
|
| Commercial assay or kit |
Dual-Luciferase kit |
Promega |
Cat# E1910 |
|
| Commercial assay or kit |
RNeasy mini kit |
Qiazen |
Cat# 74,004 |
|
| Commercial assay or kit |
QuickChange II Site-Directed Mutagenesis Kit |
Agilent |
Cat# 200,521 |
|
| Commercial assay or kit |
AAVpro Purification Kit |
Takara Bio. |
Cat# 6,675 |
|
| Commercial assay or kit |
Triglyceride Colorimetric Assay Kit |
Cayman Chemical |
Cat# 10010303 |
|
| Commercial assay or kit |
Alanine Transaminase Colorimetric Activity Assay Kit |
Cayman Chemical |
Cat# 700,260 |
|
| Commercial assay or kit |
Aspartate Aminotransferase Colorimetric Activity Assay Kit |
Cayman Chemical |
Cat# 701,640 |
|
| Commercial assay or kit |
Mouse Insulin ELISA Kit |
Crystal Chem |
Cat# 90,080 |
|
| Commercial assay or kit |
PicoSens Free Fatty Acid Quantification Kit |
BioMax |
Cat# BM-FFA-100 |
|
| Commercial assay or kit |
Β-hydroxybutyrate assay Kit |
abcam |
Cat# ab83390 |
|
| Chemical Compound, drug |
Oleic acid |
Sigma-Aldrich |
Cat# O1008 |
|
| Chemical Compound, drug |
Fenofibrate |
Santa Cruz biotechnology |
Cat# sc-204751 |
|
