KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Biological samples | ||
| Mouse olfactory epithelium | This paper | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Pentanal | Sigma-Aldrich | Cat# 110132 |
| Pentanone | Sigma-Aldrich | Cat# 471194 |
| Ethyl butyrate | Sigma-Aldrich | Cat# W242713 |
| Heptanal | Sigma-Aldrich | Cat# W254002 |
| Hexanal | Sigma-Aldrich | Cat# 115606 |
| Propanal | Sigma-Aldrich | Cat# 538124 |
| Butanal | Sigma-Aldrich | Cat# W217018 |
| Butanone | Sigma-Aldrich | Cat# W217018 |
| Hexanone | Sigma-Aldrich | Cat# 103004 |
| Ethyl acetate | Sigma-Aldrich | Cat# 270989 |
| Propyl acetate | Sigma-Aldrich | Cat# 537438 |
| Butyl acetate | Sigma-Aldrich | Cat# 287725 |
| Benzaldehyde | Sigma-Aldrich | Cat# B1334 |
| Tiglaldehyde | Sigma-Aldrich | Cat# W340707 |
| Methyl tiglate | Thermo Fisher | Cat# T024825ML |
| Methyl valerate | Sigma-Aldrich | Cat# W275204 |
| Acetophonone | Sigma-Aldrich | Cat# A10701 |
| 2-Hydroxy acetophenone | Sigma-Aldrich | Cat# H18607 |
| 4-Methyl acetophenone | Sigma-Aldrich | Cat# W267708 |
| Methyl salicylate | Sigma-Aldrich | Cat# M6752 |
| Octanal | Sigma-Aldrich | Cat# O5608 |
| Mineral oil | Sigma-Aldrich | Cat# M5904 |
| Dipropylene glycol | Sigma-Aldrich | Cat# D215554 |
| Actinomycin D | Sigma-Aldrich | Cat# A9415 |
| Triptolide | Sigma-Aldrich | Cat# T3652 |
| Anisomycin | Sigma-Aldrich | Cat# A9789 |
| Papain | Worthington | Cat# LK003178 |
| DNase I | Worthington | Cat# LK003172 |
| EBSS | Worthington | Cat# LK003188 |
| Hibernate-A | Thermo Fisher | Cat# A1247501 |
| Heat-inactivated Fetal Bovine Serum | Thermo Fisher | Cat# 16140071 |
| Propidium iodide | Thermo Fisher | Cat# P1304MP |
| PBS | Thermo Fisher | Cat# 70011044 |
| Critical commercial assays | ||
| Chromium Next GEM Single Cell 3' Kit v3.1 | 10x genomics | Cat# 1000268 |
| Chromium Next GEM Chip G Single Cell Kit | 10x genomics | Cat# 1000120 |
| Dual Index Kit TT Set A | 10x genomics | Cat# 1000215 |
| Agilent High Sensitivity DNA kit | Agilent | Cat# 5067–4626 |
| KAPA Library Quantification Kit, illumina platform, Complete kit (Universal) | Roche | Cat# KK4824 |
| Deposited data | ||
| Raw and analyzed sequencing data | This paper | GEO: GSE173947 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | JAX: 000664, RRID:IMSR_JAX:000664 |
| Mouse: OMP-IRES-GFP | The Jackson Laboratory | JAX: 006667, RRID:IMSR_JAX:006667 |
| Mouse: B6J.Cg-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze/MwarJ | The Jackson Laboratory | JAX:028865, RRID:IMSR_JAX:028865 |
| Mouse: Arrb2tm1Rjl/J | The Jackson Laboratory | JAX:011130, RRID:IMSR_JAX:011130 |
| Mouse: B6;129P2-Olfr160tm11(Olfr545)Mom/MomJ (S50(M72 locus)) | Thomas Bozza | JAX:006714, RRID:IMSR_JAX:006714 |
| Mouse: B6;129P2-Olfr545tm3(Olfr160)Mom/MomJ (M72(S50 locus)) | Thomas Bozza | JAX:006715, RRID:IMSR_JAX:006715 |
| Mouse: Olfr17tm7Mom/MomJ | Stavros Lomvardas | JAX:006669, RRID:IMSR_JAX:006669 |
| Mouse: OMP-IRES-Cre | Richard Axel | MGI:3045687 |
| Mouse: OMP-ChR2(H134R)-Venus | Thomas Bozza and Dmitry Rinberg | Li et al, 2014 |
| Software and algorithms | ||
| Python 3.6–3.8 | Python | https://www.python.org/ |
| MATLAB (2014a, 2015a) | Mathworks | https://www.mathworks.com/ |
| ScanImage 5 | Vidirio Technologies | http://scanimage.vidriotechnologies.com/display/SIH/ScanImage+Home |
| Wavesurfer 0.775 | Howard Hughes Medical Institute Janelia Research Campus | https://wavesurfer.janelia.org/ |
| Suite2p | Howard Hughes Medical Institute Janelia Research Campus | https://github.com/mouseland/suite2p |
| Adobe Illustrator | Adobe | RRID:SCR_010279, https://www.adobe.com/products/illustrator.html |
| Cell Ranger (2.2.0 for v2 and v3 data and 4.0.0 for v3.1 data) | 10x genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome |
| Scanpy 1.6.0 | Wolf et al. 2018 | https://github.com/theislab/scanpy |
| Python analysis scripts | This paper | Zenode: https://doi.org/10.5281/zenodo.5550454 GitHub: https://github.com/dattalab/Tsukahara_Brann_OSN |
| Other | ||
| Teensy 3.2 | Adafruit | Cat# 2756 |
| blue LED | Digikey | Cat# 1416–1052-6-ND |