Figure 1.

TGF-β induces EMT in human colorectal cancer cells. (a) Phase-contrast images of HT29 cells. Human colorectal cancer cell HT29 was starved in 0.5% FBS for 8 h. After starvation, cells were stimulated with TGF-β in 0.5% FBS medium for another 48 h to establish the cellular model of EMT. (b) Total RNA was extracted and detected by qRT-PCR for indicated genes in HT29 cells treated with TGF-β. All of the genes are involved in EMT. Data are shown as the mean ± SD. p < 0.05, compared with control.