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. 2021 Oct 9;121(1):157–171. doi: 10.1016/j.bpj.2021.10.008

Figure 3.

Figure 3

Experimental measurements of SynGAP/PSD-95 phase separation. (a) Sedimentation assay showing the dilute phase (marked by “S” at the top of the columns) and the condensed phase (marked by “P” at the top of the columns) distribution of PSD-95 and SynGAP mixed at different concentrations of PSD-95 and a fixed concentration of SynGAP at 90 μM. (b) Phase diagram of PSD-95 and SynGAP condensates. The open circles in phase diagram indicate no phase separation, and solid circles represent condensed-phase formation. The results enclosed in the dashed red box correspond to those from (a). The inclined dashed line indicate the dilute-solution 3:2 ratio for [SynGAP]/[PSD-95]. (c) Standard curve of Cy3-labeled PSD-95 obtained from dilute solutions. (d) Confocal images showing an indicative confocal slice of condensed droplets of each concentration of Cy3-labeled PSD-95 mixed with unlabeled SynGAP at a fixed concentration at 180 μM. (e) Heat map plot showing the PSD-95 concentrations in condensed phase at different combinations of PSD-95 and SynGAP concentrations. Each number represents the PSD-95 concentration (μM) in the condensed phase. The data enclosed in the dashed red box correspond to those from (d). a.u., arbitrary unit.