Figure 4.

Experimental trend of SynGAP/PSD-95 coexisting phases. Shown data are inferred from confocal microscopy and centrifugation measurements as described in the text. Initial (overall) concentrations ([P⁰], [S⁰]) are plotted as green data points. The phase-separated dilute phase ([P^dil], [S^dil]) are plotted as orange data points, and the condensed phase ([P^cond], [S^cond]) are plotted as blue data points. Dashed lines are tie lines connecting coexisting phases. Results are shown in linear (a) as well as log-log (b) scales for clarity, as the green data points are not visible in the linear plot in (a) because they are very close to the orange data points. Note that because the dilute-phase ([P^dil], [S^dil]) concentrations (orange data points) are all very close to the origin, the slopes of tie lines in the log-log plot in (b) are all approximately equal to 1. The diversity of the actual tie-line slopes as seen in (a) is manifested by the offsets of the log-log tie lines (different intercepts) in (b).