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. 2021 Dec 9;121(1):23–36. doi: 10.1016/j.bpj.2021.12.009

Figure 4.

Figure 4

Effect of the ramping parameters on the shape transition. Experimental data are shown as boxplots with superimposed individual gray data points. Outliers are marked using “+” symbols. Simulation results are plotted as red symbols. Cells that show pronounced rotations during the shape transitions are highlighted as stars. (a) Transition time Δt for an upward ramp as a function of the total pressure difference at a constant ramp duration of tramp = 0.5 s. For Δp=500mbar and Δp=650mbar, the velocities in the simulations are Δv4.7mms1 and Δv6.2mms1, respectively. (b) Transition time for different ramping duration for an upward ramp with Δp=500mbar, corresponding to Δv4.7mms1 in the numerical simulation. In (c), all individual measurements for the upward and downward ramp directions are combined. The inset shows the experimentally observed transition times normalized by the mean transition time Δt¯. (d) Transition length Δx as a function of the ramping direction, combining all individual measurements, similar to (c). The individual measurements of Δt and Δx are shown Figs. S5 and S6 in the Supporting Material, respectively.