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. 2021 Dec 1;13(2):162–172. doi: 10.1111/1759-7714.14235

FIGURE 5.

FIGURE 5

NUCKS1 acted as a target of miR‐433‐3p. (a) The alignment of miR‐433‐3p with the 3′UTR of NUCKS1. (b, c) Dual‐luciferase reporter assay was executed to analyze the luciferase intensity of the psiCHECK‐2 vector carrying NUCKS1 3′‐UTR‐WT or NUCKS1 3′‐UTR‐MUT in NSCLC cells transfected with miR‐NC or miR‐433‐3p mimic. (d, e) RT‐qPCR and western blotting were executed to analyze the levels of NUCKS1 mRNA and protein in NSCLC cells transfected with miR‐NC or miR‐433‐3p mimic. (f, g) RT‐qPCR and western blotting were carried out to assess the levels of NUCKS1 mRNA and protein in NSCLC tissues and paracarcinoma tissues. (h) Correlation of NUCKS1 mRNA and miR‐433‐3p in NSCLC tissues was analyzed by Pearson's correlation analysis. (i, j) RT‐qPCR and western blotting were performed to examine the levels of NUCKS1 mRNA and protein in NSCLC cells and BEAS‐2B cells. (k, l) Levels of NUCKS1 mRNA and protein in NSCLC cells transfected with si‐NC, si‐circ_0008037, si‐ circ_0008037 + anti‐miR‐NC, or si‐circ_0008037 + anti‐miR‐433‐3p were detected by RT‐qPCR and western blotting. (m) Correlation of NUCKS1 mRNA and circ_0008037 in NSCLC tissues was analyzed by Pearson's correlation analysis. *P < 0.05