Fig. 5. Holo-Lf-mediated amyloidogenic processing of APP requires ARF6 and the Rab11-positive recycling endosome.

A Amyloidogenic processing of APP induced by holo-Lf (500 nM; 2 h) in wt-APP⁶⁹⁵ SH-SY5Ys pre-treated with control non-target or ARF6 RNAi (20 nM; 48 h) was measured by sAPPβ and Aβ levels in the media. B, C wt-APP⁶⁹⁵ SH-SY5Ys were reverse transfected with control non-target or Rab4a RNAi and/or forward transfected with Rab11a RNAi (20 nM; 48 h) before addition of holo-Lf (500 nM; 2 h). Extracellular sAPPβ and Aβ (B) as well as intracellular Aβ (C) protein levels were quantified. Data are mean ± SEM of three experiments performed in triplicate and depicted as fold change compared with levels derived from control non-target cells. Statistical analysis was by two-way ANOVA compared to untreated non-targeting control, ****p < 0.0001 or holo-Lf-treated non-targeting control, ^^^^p < 0.0001.