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. 2020 Nov 3;26(10):5592–5607. doi: 10.1038/s41380-020-00926-w

Fig. 1. Validation of functional miRNAs targeting FERMT2 3′UTR.

Fig. 1

a Relative positions of miRNA-target sites on FERMT2 3′UTR. The target site created by the rs7143400-T allele, which is associated with AD risk, is shown in red. b Luciferase activity of FERMT2 3′UTR carrying either the rs7143400-G or the rs7143400-T allele in HEK293 cells co-transfected with a nontargeting miRNA (miR-NT) or 5 miRNA mimics. Data are expressed relative to the miR-NT. c RFLP genotyping of HeLa and HEK293 cell lines edited or not for the rs7143400 via CRISPR–Cas9 (Supplementary Fig. 1). d Endogenous FERMT2 expression levels were assessed by Western blot using indicated cell extracts following transient transfection with a nontargeting miR (miR-NT) or with the indicated miR for 72 h. e WB quantifications from three independent experiments as in d Data given in mean ± SD. *p < 0.05, nonparametric test compared to miR-NT condition.