Fig. 1.

(A) Example images of the GFP signal patterns identified in cultures of HEK293T cells transfected with ACE2-GFP incubated with vehicle or RBD (10 μg/mL) for 40 min. Objective magnification: 60×. (B) Example images of HEK293T cells transfected with ACE2-GFP incubated with vehicle or RBD 10 μg/mL for 40 min and immunostained against RBD. For nuclei visualization cells were co-stained with Höescht. The asterisk in merged image of RBD-treated cells shows that a non-transfected cell is negative for RBD. Arrowheads point at colocalization of ACE2-GFP and RBD signal. Objective magnification: 63×.