Fig. 3.

(A) GFP patterns abundance (%) in cultures of HEK293T cells transfected with ACE2-GFP alone (ACE2-GFP) or co-transfected with AT1R (ACE2-GFP + AT1R) incubated with vehicle or RBD (10 μg/mL) for 40 min. Association between treatments and GFP patterns frequency in RBD-treated groups was evaluated by Chi-square test (χ² = 1.237, P_chi = 0.54; n = 30–50 cells per condition). (B) GFP patterns abundance (%) in cultures of HEK293T cells co-transfected with ACE2-GFP and AT1R (ACE2-GFP + AT1R) co-incubated with vehicle or RBD and vehicle (+Veh), telmisartan (+Telm, 10 μM) or angiotensin II (+Ang II, 1 μM) for 40 min. Telmisartan and Ang II were added to the culture medium 5 min prior to RDB. Association between treatments and GFP patterns frequency in RBD-treated groups was evaluated by Chi-square test (χ² = 17.89, P_chi < 0.01; * indicates different from the expected frequency of the corresponding GFP pattern, P < P_corr = 0.004; Somers' D = 0.24; n = 35–47 cells per condition).