Fig. 4.

GFP patterns abundance (%) in cultures of HEK293T cells transfected with ACE2-GFP alone (ACE2) or co-transfected with B2R (+B2R) incubated with vehicle or RBD (10 μg/mL) for 40 min. In +B2R + BK condition, cells were co-incubated with BK plus vehicle or BK plus RBD (BK, 0.5 μM) for 40 min. BK was added to the culture medium 5 min prior to RBD. Association between treatments and GFP patterns frequency in RBD-treated groups was evaluated by Chi-square test (χ² = 47.29, P_chi < 0.0001; * indicates different from the expected frequency of the corresponding GFP pattern, P < P_corr = 0.006; n = 26–48 cells per condition).