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. 2021 Dec 31;9:791187. doi: 10.3389/fcell.2021.791187

FIGURE 5.

FIGURE 5

MIF can be transferred from NPC cells to Møs via exosomes. (A) Representative electron microscopy images of NPC-exos and Normal-exos. (B) Uptake of exosomes in macrophages by confocal microscopy. Exosomes are derived from the serum of NPC patients. Blue: DAPI staining; green: PKH67-labeled exosomes. Scale bar:20 um. (C) Validation of serum-derived exosomes markers (D–F) WB analyses of MIF and GPX4 expression in NPC cell lines, NP69 cells and macrophages (*p < 0.05, **p < 0.01, ***p < 0.001, t-test). (G,H) Analysis of the expression level of MIF in exosomes of NPC and normal cells (t test). (I,J) Analysis of the expression level of MIF in exosomes in serum from three cases of nasopharyngeal carcinoma and normal controls (t test). (K) CM without exo: the supernatant derived from the CNE2 control and transfected knockdown MIF lentivirus, and the exosomes were removed. exo: Exosomes. All graphs show the mean ± SEM of at least three independent experiments.