Table 1.

Sequences of oligonucleotides used in mutagenesis.

Primer	Sequence (5′ to 3′)	References
SpxKO-F1-BamHI	ATGGATCCCATCTTTATTATAAGCACCCTCAC	33
SpxKO-R1	GAGAGTTATCATTATGACTCAGGATTGCAATC ACGCGCAATC	33
SpxKO-F2	TTGCGCGTGATTGCAATCCTGAGTCATAATGA TAACTCTCCTTC	33
SpxKO-R2-SacI	GCGAGCTCATTGGTATCGAAGAGGTCATTGC	This study
Spx-inside-F	ACCATGGAGTAGACTCAACTGG	33
Spx-inside-R	ATGGATAACACTCCATTCCTTG	33
SpxB-EcoRI-F	ATGAATCCGGATTGCTCCGATCTTTTCA	This study
SpxB-BamHI-R	CTGGATCCCGAGCTTGCTCGCTTTTTAT	This study