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. 2022 Jan 13;12:698. doi: 10.1038/s41598-021-04700-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative 2D gels of PI protein spots obtained from shoot tips of Catongo and TSH1188 inoculated and non-inoculated [mock inoculated control] at 72HAI and 45DAI post-infection with M. perniciosa. Total protein extract [500 μg] were focused on IPG strips [13 cm], with pH ranging from 3 to 10. NL separated by SDS-PAGE [12.5%] and stained with CBB G-250. The green highlight indicates putative PI isoform spots. (a) Catongo 72 HAI non-inoculated [mock inoculated control]. (b) Catongo 72 HAI. (c) TSH1188 72 HAI non-inoculated [mock inoculated control]. (d) TSH1188 72 HAI. (e) Catongo 45 DAI non-inoculated [mock inoculated control]. (f) Catongo 45 DAI. (g) TSH1188 45 DAI non-inoculated [mock inoculated control]. (h) TSH1188 45 DAI.