Table 1.

Data collection and refinement statistics for lysozyme and glucose isomerase delivered in beef tallow injection matrix.

Data collection	Lysozyme	Glucose isomerase
Diffraction source	11C beamline, PLS-II	11C beamline, PLS-II
Wavelength (Å)	0.9794	0.9794
Temperature (K)	298.5	298.5
Detector	Pilatus 6 M	Pilatus 6 M
Collected images	80,000	80,000
Hit images	21,922	27,380
Indexed images	20,234	7795
Indexed pattern	23,090	7976
Space group	P43212	I222
a, b, c (Å)	79.45, 79.45, 38.47	94.33, 99.92, 103.31
α, β, γ (°)	90, 90, 90	90, 90, 90
Resolution range (Å)	80.00–1.55 (1.60–1.55)	72.46–1.60 (1.66–1.60)
No. of unique reflections	18,428 (1809)	64,497 (6368)
Completeness (%)	100.0 (100.0)	100.0 (100.0)
Redundancy	1257.8 (667.4)	250.9 (167.6)
SNR	10.63 (1.87)	4.79 (1.52)
CC	0.9941 (0.6243)	0.9656 (0.5348)
CC*	0.9985 (0.8767)	0.9912 (0.8348)
Rsplit	6.65 (58.61)	16.70 (70.82)
Wilson B factor (Å2)	27.02	22.61
Refinement
Resolution range (Å)	56.14–1.55 (1.60–1.55)	71.80–1.80 (1.66–1.60)
Rwork	0.1678 (0.2705)	0.1681 (0.2870)
Rfree	0.1874 (0.3274)	0.1862 (0.2930)
R.m.s. deviations
Bonds (Å)	0.013	0.007
Angles (°)	1.519	0.914
Average B factors (Å2)
Protein	28.80	21.78
Ligand	33.87	11.72
Water	38.04	33.89
Ramachandran plot
Most favoured (%)	98.43	96.34
Allowed (%)	1.57	3.40
Outliers (%)	0.00	0.26

Values for the outer shell are given in parentheses.

R_split = $\left(\raisebox{1ex}{$1$}\!\left/ \!\raisebox{-1ex}{$\sqrt{2}$}\right.\right)·\frac{{\sum }_{\mathit{hkl}}\left|I_{\mathit{hkl}}^{\mathit{even}}-I_{\mathit{hkl}}^{\mathit{odd}}\right|}{\frac{1}{2}\left|I_{\mathit{hkl}}^{\mathit{even}}-I_{\mathit{hkl}}^{\mathit{odd}}\right|}$.

R_free was calculated as R_work using a randomly selected subset (10%) of unique reflections not used for structure refinement.