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. 2022 Jan 13;12:667. doi: 10.1038/s41598-021-04560-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ABD-GCSF protein. (a) Protein expression: M: Protein Mw marker; #1, 3: Recombinant bacterial lysates before induction; #2, 4: Recombinant bacterial lysates after induction. (b) Protein purification: #1: Initial sample (IS); #2: Flow through (FT) sample; #3: Washing sample; M: Protein Mw marker; #4–8: Eluted samples. (c) Western blotting: M: Protein Mw marker; #1, 2: Bacterial lysates before and after induction; #3, 4: Eluted proteins. The full-size original gels and the blot are presented in supplementary Fig. S1a,b,c, respectively.