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. 2022 Jan 13;12:667. doi: 10.1038/s41598-021-04560-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Size exclusion chromatography of Filgrastim and ABD-GCSF. Molecular weight marker includes the following proteins: (1) vitamin B12 (1.350 kDa); (2) horse myoglobin (17 kDa); (3) chicken ovalbumin (44 kDa); (4) bovine c-globulin (158 kDa); (5) bovine thyroglobulin (670 kDa); (5). Last peak obtained at retention time of 22 min represents excipients from the dilution buffer.