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. 2022 Jan 13;5:51. doi: 10.1038/s42003-022-03005-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The RORA wild type (WT) or RORA null (RORA^−/−) backgrounds were used to generate either control (CTL) or STZ-induced diabetic (STZ) pregnant dams, and the amygdala neurons and/or tissues from subsequent male offspring were isolated for further analysis. a–d The amygdala tissues were isolated from 7- to 8-week-old male offspring for analysis. a The mRNA levels by qPCR, n = 4. b The quantitation of protein levels, n = 5. c The representative pictures for western blotting for (b). d CSF E2 levels, n = 9. e Estrogen receptor activity, n = 9. f In vivo superoxide anion release, n = 5. g, h The amygdala neurons were isolated on an embryonic day (E18) from the above treatment for immunostaining. g Quantitation of 8-oxox-dG staining, n = 5. h Representative pictures for 8-oxo-dG staining (green) and DAPI staining for nuclei (blue). ***, P < 0.0001; **, P < 0.001; *, P < 0.01; N/S, no significance. Data were expressed as mean ± SEM.