Fig. 2. ZC3H15 is essential for cell proliferation, migration and invasion of GBM cells.

A Western Blot and qRT-PCR assay were performed to prove the knockdown of ZC3H15. B, C The cells viability and BrdU incorporation were detected by MTT and BrdU assay. BrdU positive cells were counted. D ZC3H15-knockdown and control cells were plated on glass slides and stained with anti-Paxillin and anti-actin antibodies. DAPI was used for nuclear staining. E The expressions of metastasis-associated proteins were evaluated through western blot analysis in ZC3H15-knockdown and control cells. Tubulin was used as a control for normalization. F The effect of ZC3H15 knockdown on cell migration was detected by transwell assay, the migrated cells were counted and analyzed. G Western blot and RT-PCR analysis were performed to detect the expression of ZC3H15 in the indicated cells. H, I The proliferation abilities of indicated cells were analyzed by MTT and BrdU assays. BrdU positive cells were counted and analyzed. J Indicated cells were plated on glass slides and stained with anti-Paxillin and anti-actin antibodies. DAPI was used for nuclear staining. K The expressions of metastasis-associated proteins were evaluated through western blot analysis in GBM cells. Tubulin was used as a control for normalization. L The migration ability of indicated cells was detected by transwell assay. All data were expressed as mean ± SD. Student’s t test was performed to analyzed significance. *P < 0.05, **P < 0.01, ***P < 0.001.