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. 2022 Jan 13;8:5. doi: 10.1038/s41523-021-00375-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a QPCR validation of candidate genes in sorted HCC38 cell types. b Functional screen for candidate genes necessary for methylcellulose colony formation after transient siRNA knockdown in BT549 cells. Target gene knockdown was validated by QPCR. Statistics by one-way ANOVA with Dunnett’s test. *P < 0.05. c Representative immunoblot of lysates from sorted HCC38 cells. β-actin is shown as a loading control. Molecular weight markers are indicated in kilodaltons. d Representative FACS density plot of the live, CD49f⁺ SUM149 cells according to their cell surface EpCAM and αvβ3 status. e QPCR validation of candidate genes in sorted SUM149 cells. a, e Samples were run in duplicate with GAPDH as a loading control. Expression is shown relative to the EpCAM^Low/αvβ3⁻ cells (dashed lines). a, b, e Data represent the mean ± s.e.m. a–e n = 3 independent experiments. See also Supplementary Fig. 4.