Table 3.
Time (days) | Protein/ (mg/L) | Amino acid nitrogen/(mg/100 g) | TTA /(g/L) | pH | Reducing sugar/(mg/mL) | TPC /(mg/L) | TFC /(mg/L) | Ascorbic acid/(mg/100 g) | SOD activity /(U/mL) |
---|---|---|---|---|---|---|---|---|---|
0 | 0.27 ± 0.01a | 2.43 ± 0.02e | 1.84 ± 0.16f | 3.81 ± 0.02a | 25.91 ± 0.50 h | 42.05 ± 0.94d | 27.19 ± 1.11a | 152.81 ± 1.80a | 27.73 ± 1.42a |
2 | 0.24 ± 0.03a,b | 2.44 ± 0.06e | 2.15 ± 0.18f | 3.74 ± 0.04b | 145.46 ± 0.69a | 51.10 ± 1.05b | 27.37 ± 0.65a | 51.76 ± 1.78b | 20.68 ± 1.04b |
4 | 0.27 ± 0.04a | 4.50 ± 0.02d | 3.71 ± 0.20e | 3.66 ± 0.02c | 99.80 ± 0.31b | 56.74 ± 0.73a | 20.74 ± 0.64c | 33.98 ± 0.22c | 22.73 ± 3.15b |
3 | 0.25 ± 0.02a,b | 5.16 ± 0.29c | 3.91 ± 0.41d,e | 3.62 ± 0.00d | 81.07 ± 0.36c | 44.22 ± 1.46c | 16.64 ± 1.94d | 19.46 ± 1.38d | 27.73 ± 1.42a |
8 | 0.23 ± 0.01b | 6.29 ± 0.78a,b | 6.03 ± 0.17a | 3.60 ± 0.01d | 63.87 ± 3.04d | 39.20 ± 0.18e | 23.73 ± 1.40b | 17.16 ± 1.53d | 23.35 ± 1.37b |
38 | 0.21 ± 0.02b | 6.44 ± 0.11a | 4.24 ± 0.36c,d | 3.61 ± 0.02d | 55.97 ± 1.13e | 35.69 ± 0.24f | 12.55 ± 0.77e | 12.92 ± 0.76e | 16.59 ± 3.36c |
68 | 0.12 ± 0.01c | 5.90 ± 0.03b | 4.95 ± 0.10b | 3.63 ± 0.01c,d | 36.28 ± 0.63f | 34.06 ± 1.22f | 7.46 ± 0.36f | 9.38 ± 1.94e | 10.68 ± 1.72d |
98 | 0.06 ± 0.01d | 4.95 ± 0.05c,d | 4.40 ± 0.06c | 3.61 ± 0.02d | 29.49 ± 0.47 g | 25.39 ± 1.17 g | 3.56 ± 0.12 g | 4.29 ± 0.05f | 9.09 ± 1.04d |
Values are means ± standard deviation (SD). Different letters in the same column represent significant differences (p < 0.05)
aTTA total titration acid; TPC total phenolic content; TFC total flavonoid content; SOD superoxide dismutase