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. 2021 Dec 22;17(1):96–109. doi: 10.1016/j.stemcr.2021.11.009

Figure 1.

Figure 1

Characterization of hiPSC-cortical neuron maturation via phase microscopy, immunocytochemistry, and flow cytometry

(A) Timeline detailing the differentiation process from hiPSCs to cortical neurons.

(B) Phase images depicting cell morphology on day 7 (left), day 14 (middle), and day 40 (right).

(C and D) Immunocytochemistry of cortical neurons stained for β-III tubulin and ctip2 (day 40) in (C) and for β-III tubulin and GFAP (day 28) in (D).

(E) Graph of flow cytometry data on days 0, 14, and 28 illustrating neuron maturation by the expression of layer V cortical neuron marker ctip2, neuronal marker β-III tubulin, and glial cell marker GFAP. Error bar: SEM. N = 3 independent experiments. Scale bar, 50 μm.