Figure 3.

Electrophysiological characterization of hiPSC-cortical neurons via whole-cell patch clamp

(A) Representative traces of spontaneous firing (top), depolarization-induced repetitive firing (middle), and Na⁺ and K⁺ currents (bottom) recordings from day 40 neurons.

(B and C) Percentage of cells experiencing spontaneous firing (B) and repetitive firing (C), indicating maximal activity at day 40.

(D and E) Quantification of action potential voltage (D) and mean inward Na⁺ current (E), indicating maximum level at day 40.

(F and G) Quantification of resting membrane potential (F) and mean K⁺ outward current (G). Statistical analysis was performed using one-way ANOVA followed by Tukey's test; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Error bar: SEM. N = 9 cells total from three independent experiments.