Figure 6.

Confirmation of excitatory and inhibitory character in mature and immature iPSC-cortical neurons

(A and B) Immunocytochemistry of cortical neurons stained for VGlut1 (green) and GABA (red) indicating the neurons expressed both excitatory and inhibitory markers at day 21 (A), but were typically glutamatergic at D 40 (B).

(C) Quantitative MEA analysis of LTP induction for day 21 and day 35 neurons. LTP induction in day 21 neurons did not persist after 1 h when dosed with vehicle control but did so when dosed with 100 μM picrotoxin prior to LTP induction, while it was similar to those observed in innate day 35 neurons without any picrotoxin addition. All the data were normalized to the day 21 undosed baseline. Statistical analysis was performed using one-way ANOVA followed by Tukey's test; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Error bar: SEM. Scale bar, 50 μm, N = 10 electrodes from three individual experiments.