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. 2021 Dec 30;17(1):159–172. doi: 10.1016/j.stemcr.2021.11.014

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Regional mapping and quantification of two-neuron tracing to nigral transplants

(A) Correlation between host input to the SN and the A9-type neurons in the hESC-derived transplant (TX), log-log transformed plot (R = 0.7; p < 0.01).

(B) Corresponding correlation between the input to the host VTA and A10-type neurons in the TX (R = 0.4; p = 0.07).

(C) Plotting of the number of labeled afferent neurons in each region compared between the two tracing groups and the intact dopamine system of TH-Cre⁺ rats. In each brain structure (one line), the top bars (darker red and blue) represent the TH-Cre⁺ intact rats, and the lower bars (lighter red and blue) represent the hESC transplanted animals. The centerline's left side contains the animals where tracing was initiated from the PFC (A10-type neurons) and the right side with tracing initiated from the Str (A9-type neurons). ^∗A significant difference from A9-type neurons.

(D–F) Mapping of all traced neurons into a 3D brain atlas. (D) Individual monosynaptic afferent neurons to A9-type (SN) neurons. (E) Individual monosynaptic afferent neurons to A10-type (VTA) neurons. (F) The traced cells are aggregated into 3D heat maps for A9-type neurons, compared with A10-type neurons and connectivity traced from an ectopically placed transplant in the Str.