Figure 2.

Reactivation of X-linked genes from the Xi in partially reprogrammed iPSCs

(A) Generation of iPSCs with different pluripotency levels (Low-K, Mid-K, and High-K) by SeVdp(fK-OSM) in the presence of different Shield1 concentrations.

(B) Expression kinetics of pluripotency markers in partially reprogrammed iPSCs. HAT-bsMEFs were reprogrammed under the Low-K and Mid-K conditions. The expression levels of indicated genes were determined by qRT-PCR at indicated days of reprogramming.

(C) Relative abundances of allele-specific transcripts of Lamp2 and Atrx in HAT-bsMEFs reprogrammed under the Low-K and Mid-K conditions. The same cells prepared in (B) were analyzed for qRT-PCR using TaqMan probes.

All data represent mean ± SEM of at least three independent experiments. ^∗p < 0.05 and ^∗∗p < 0.01 versus HAT-bsMEFs (day 0).