Figure 4.

Transcriptional reactivation from the Xi and Xist expression in single cells

(A) Flowchart of single-cell analysis to detect Xist expression and allele-specific Lamp2, Atrx, and Hdac6 transcripts.

(B and C) Patterns of allele-specific gene expression in single cells. Expression of Hdac6, Lamp2, and Atrx from the Xi in single cells was determined using qRT-PCR with TaqMan probes. Xist expression was also determined from same cDNAs. Cells were categorized into indicated groups by expression patterns of Hdac6 and Lamp2 or Atrx (B) or Hdac6 and Xist (C).

(D) Cells that showed Hdac6, Lamp2, and Atrx reactivation with Xist expression. The numbers indicate percentages of cells that expressed Hdac6, Lamp2, or Atrx from the Xi in populations of Xist-expressing cells.

Raw data are shown in Figure S5.