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. 2021 Dec 16;17(1):110–126. doi: 10.1016/j.stemcr.2021.11.007

Figure 1.

Figure 1

APOE-isogenic iPSCs are differentiated to iAstrocytes

(A) Schematic representation of the iAstrocyte differentiation protocol.

(B) Heatmap of top 100 differentially expressed proteins between NPCs and iAstrocytes selected by fold change.

(C) PCA of proteomic data from NPCs and iAstrocytes of all genotypes. Three biological replicates were analyzed.

(D) Western blot of astrocyte marker proteins IQGAP1 and S100A6 in APOE-isogenic NPCs and astrocytes.

(E) Immunocytochemistry of astrocyte markers S100β and GJA1 in APOE-isogenic iAstrocytes at day 44. Scale bar: 150 μm.

(F) Proliferation rate of APOE-isogenic iAstrocytes at different time points in culture.

See also Figure S1.