Figure 5.

hM4Di-NS/PC-transplanted mice showed locomotor functional changes induced by the inhibition of graft neuronal activity

(A) Schematic representing the time schedule of the in vivo experiment.

(B and C) BMS scores before CNO administration, after CNO administration, and after CNO washout. The scores at 28 days after SCI (B) and 70 days after SCI (C) are presented (mCherry-TP group, n = 18; hM4Di-TP group, n = 20).

(D) Comparison of BMS scores in CNO-administered mice on day 70 (PBS group, n = 20; hM4Di-TP group, n = 20).

(E) Raw data of paw angles before CNO administration, after CNO administration, and after CNO washout for mCherry-TP mice (left) and hM4Di-TP mice (right).

(F) Quantification of paw angles before CNO administration, after CNO administration, and after CNO washout (mCherry-TP group, n = 9; hM4Di-TP group, n = 12).

(G) Quantification of the paw angle changes before and after CNO administration in each animal (mCherry-TP group, n = 9; hM4Di-TP group, n = 12).

(H) Raw stride-length data before CNO administration, after CNO administration, and after CNO washout for mCherry-TP mice (left) and hM4Di-TP mice (right).

(I) Quantification of stride length before CNO administration, after CNO administration, and after CNO washout (mCherry-TP group, n = 9; hM4Di-TP group, n = 12).

(J) Quantification of the stride length changes before and after CNO administration in each animal (mCherry-TP group, n = 9; hM4Di-TP group, n = 12). ^∗p < 0.05, ^∗∗p < 0.01, and N.S., not significant (not displayed in the figures [B, C, F, and I]), according to the Wilcoxon signed rank test following Friedmann test (B, C, F, and I) and the Mann-Whitney U test (D, G, and J). The data are presented as the mean ± SEM (B–D, F, G, I, and J). All multiple testing data analyses were followed by Bonferroni correction.