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. 2022 Jan;192(1):121–129. doi: 10.1016/j.ajpath.2021.09.009

Figure 3.

Figure 3

A–D: Histopathology (hematoxylin and eosin) (A), immunohistochemistry (B and C), and RNAscope in situ hybridization (D) of male reproductive organs associated with EBOV infection. A: Acute perivascular posthitis with microthrombus (arrow) is observed in a male macaque 8 days after exposure. Scale bar: 100 μm. B: Immunohistochemistry shows that many macrophages, stromal cells, and/or circulating monocytes in the lamina propria of a penis (serial section from panel A) are strongly EBOV-VP40 positive. Scale bar: 100 μm. C: Immunohistochemistry shows that a few number (cell accounts)/amount (quanity of mixture) of epididymal tubular epithelial cells (arrows) and intraluminal mixture of secretory material and sperm (arrowheads) are EBOV-VP40–antigen positive from a male macaque 6 days after exposure. Scale bar: 100 μm. D: RNAscope in situ hybridization shows that a few number/amount of tubular epithelial cells (arrows) and intraluminal mixture of secretory material and cell debris (arrowheads) in a seminal versicle are EBOV–genomic RNA positive from a male macaque 6 days after exposure. Scale bar: 50 μm.