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. 2021 Dec 31;12:816086. doi: 10.3389/fpls.2021.816086

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Copyright © 2021 Peng, Wang, Zhang, Jiang, Zhao, Shahid, Bai, Hao, Peng, Gao, Su and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein localization, binding and regulation abilities of EjRAVs on FT and SOC1 homologs in loquat. (A) Subcellular localization of EjRAV1/2 proteins expressed in tobacco epidermal cells. (B) EjRAV1/2 regulation on EjFTs and EjSOC1s expressions in N. benthamiana leaves by using the dual-luciferase (LUC) system. Relative renilla (REN) luciferase activity was used as an internal control. Data represent means ± standard error from three replicates. **P < 0.01 and NS indicate significance and non-significance, respectively. (C,D) Binding ability of EjRAV1 to P1 of EjFT1 promoter in EMSA assays. P1, P2, P3, P4, and P5 are presumptive RAV protein binding sites on promoter of EjFT1 at (−1583 to −1579 bp), (−1059 to −1054 bp), (−1033 to −1029 bp), (−782 to −778 bp), and (−73 to −66 bp). Unlabeled probe could reduce binding ability of EjRAV1 to P1 in (D). (E) Binding ability of EjRAV1 and EjRAV2 to segment3 (−1118 to −994 bp) of EjFT2 promoter in Y1H assays.