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. 2022 Jan;192(1):56–71. doi: 10.1016/j.ajpath.2021.09.005

Figure 4.

Figure 4

METTL3-depleted hepatocytes exhibit reduced binuclearity and increased polyploidy. A: Fluorescence microscopic pictures of liver sections from wild-type (WT) and liver-specific METTL3 knockout (M3LKO) mice stained with β-catenin antibody for membrane (red) and Hoechst dye for nuclei (blue), respectively. Representative images of 10-week–old mouse livers are shown. B: Binucleate hepatocytes of 2-, 5-, and 10-week–old mice were counted in several fields and the relative populations in WT and M3LKO (3 mice per genotype per age group) mice are presented. C and D: DNA content of freshly isolated liver nuclei was determined by flow cytometry after staining with propidium iodide (PI). The percentage of nuclei with 2c, 4c, 8c, and 16c DNA content was determined using FlowJo software version 9.9.6 (Beckton Dickinson, Ashland, OR). Representative ploidy profiles are shown for 10-week–old WT and M3LKO mice (C), and the percentage of each ploidy population is summarized at 5 and 10 weeks (D). n = 3 mice per genotype. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (t-test). Scale bars: 50 μm (A, top row); 25 μm (A, bottom row).