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Chronic high-fat diet–induced beta-cell apoptosis. A: Three-month–old mice were put on a high-fat diet (HFD) or normal chow (NC) for 9 and 14 months. Sections of pancreas were stained with insulin (red) and TUNEL (green). Blue, DAPI. Arrows: TUNEL-positive cells. B: Quantification of the TUNEL-stained islets in 14-month mice using ImageJ version 2.0 software. n = 5. Scale bar = 100 μm.
