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Prolonged exposure to palmitic acid treatment reduces cell survival/growth in multiple cell lines. In mouse embryonic fibroblasts (mEF; top row), as well as three beta-cell cell lines (bottom three rows), exposure to palmitic acid for 24 hours (left column) and 48 hours (right column) induced loss of cell viability/growth potential as measured with MTT assay. Experiments were repeated multiple times. n = 3. ∗P < 0.05 compared to vehicle-treated samples.
