Figure 7.

Induction of mTOR-S6K leads to down-regulation of AKT phosphorylation due to exposure to palmitic acid (PA). A: INS-1 cells were treated with PA or PA+rapamycin and analyzed for phospho-AKT (P-AKT), total AKT (tAKT), and phospho-S6K (P-S6K). B: INS-1 cells treated with PA or vehicle (Veh) were analyzed for Raptor. C: Proposed role of palmitic acid on loss of beta-cell function via S6K-regulated AKT phosphorylation. Each lane represents an independent cell culture experiment or mouse. Densitometry quantification is provided for P-AKT/AKT, P-S6K/GAPDH, and Raptor/actin.