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. 2022 Jan;192(1):160–177. doi: 10.1016/j.ajpath.2021.10.003

Figure 4.

Figure 4

Altered myofiber isotypes in Sigmar1−/− mice muscles. A and C: Representative immunofluorescence images for anti-myosin types (types I, IIA, IIB, and IIX; left to right in red) and wheat germ agglutinin (in green) stained gastrocnemius (Gastro) and soleus (Sol) muscle sections from wild-type (Wt) and Sigmar1−/− mice. B and D: Bar graphs represent the percentage of the myofibers positive for myosin types I, IIA, IIB, and IIX in Gastro and Sol muscle sections isolated from 9- to 10-month–old Wt and Sigmar1−/− mice. The absolute number of myofibers positive for each myosin type was quantified and expressed as a percentage of total myofibers per microscopic field. The percentage numbers of myosin types I, IIA, IIB, and IIX positive myofibers were quantified from 3000 to 4000 myocytes for Gastro and from 800 to 900 myocytes for Sol muscles for each group. Dots in the bar graphs represent individual values for each Wt and Sigmar1−/− mouse. P values were determined using a two-tailed unpaired t-test. Data are expressed as means ± SEM (B and D). n = 3 mice per genotype. ∗P < 0.05, ∗∗P < 0.01. Scale bars = 200 μm (A and C).