Skip to main content
. 2022 Jan;192(1):160–177. doi: 10.1016/j.ajpath.2021.10.003

Figure 8.

Figure 8

Increased fibrosis in the skeletal muscles of Sigmar1−/− mice compared with wild-type (Wt) mice. A–E: Masson trichrome staining was conducted to assess interstitial fibrosis in muscle sections from Wt and Sigmar1−/− mice. A–E: Left panels: Representative micrographs of trichrome-stained histologic cross-sections of gastrocnemius (Gastro; A), quadriceps (Quad; B), tibialis anterior (TA; C), soleus (Sol; D), and extensor digitorum longus (EDL; E) muscle sections from Wt and Sigmar1−/− mice. Images represent 8 to 12 high-magnification microscopic fields (×20) per mice muscle sections for Gastro, Quad, and TA muscles, and from 2 to 6 high-magnification microscopic fields (×20) for Sol and EDL muscles per mice. A–E: Right panels: Box plots represent quantifications of percentage fibrosis area corresponding to total muscle section areas in trichrome-stained whole muscle areas in Wt and Sigmar1−/− mice skeletal muscles. Fibrosis areas (percentages) were quantified on 8 to 12 high-magnification microscopic fields (×20) per mice muscle sections for Gastro, Quad, and TA muscles, and on 2 to 6 high-magnification microscopic fields (×20) for Sol and EDL muscles per mice. P values indicate statistical significance between Wt and Sigmar1−/− mice. Boxes represent interquartile ranges, lines represent medians, and whiskers represent ranges. P values were determined by unpaired t-test. n = 5 individual mice at the age of 9 to 10 months per genotype per muscle (A–E). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. Scale bars = 50 μm (A–E).