Table 3.

Changes in Enzymes Associated with Cholesterol Biosynthesis

Symbol	Protein name	wt WD/wt NC	ko WD/ko NC
CYP51A1	Cytochrome P450 family 51 subfamily A1	−5.051	−4.785
DHCR24	24-Dehydrocholesterol reductase	−1.842	−2.212
DHCR7	7-Dehydrocholesterol reductase	−2.660	−1.988
FDFT1	Farnesyl-diphosphate farnesyltransferase 1	−2.041	−1.727
FDPS	Farnesyl diphosphate synthase	−7.092	−4.274
HMGCR	3-Hydroxy-3-methylglutaryl-CoA reductase	−1.681	−2.000
HMGCS1	3-Hydroxy-3-methylglutaryl-CoA synthase 1	−2.833	−6.711
HSD17B7	Hydroxysteroid 17-beta dehydrogenase 7	−2.294	−1.880
LSS	Lanosterol synthase	−2.273	−2.288
MSMO1	Methylsterol monooxygenase 1	−4.717	−5.102
MVD	Mevalonate disphosphate decarboxylase	−3.846	−3.247
MVK	Mevalonate kinase	−4.016	−2.967
NSDHL	NAD(P) dependent steroid dehydrogenase-like	−4.785	−3.460
PMVK	Phosphomevalonate kinase	−2.577	−1.894
SQLE	Squalene epoxidase	−6.061	−4.292
TM7SF2	Transmembrane 7 superfamily member 2	−2.008	−1.546

Abbreviated cholesterol biosynthesis pathway (enzymes are in parentheses): acetoacetyl-CoA/acetyl-CoA → HMG-CoA→ mevalonate (MVK)→isopentenyl-pyrophosphate (IPP, FDPS) → squalene (SQLE/LSS) → lanosterol → cholesterol (for complete list see Supplemental Figure S4).

ko, knockout; NC, normal chow; WD, Western diet; wt, wild-type.