Table 1. Unbinding Energy Landscape Parameters of the Anticalin:CTLA-4 Complex under Different Pulling Geometries.
| anchor point on anticalin | log(k0) (DHS) | log(k0) (BE) | Δx⧧ [nm] (DHS) | Δx⧧ [nm] (BE) | ΔG⧧ [kBT] (DHS) |
|---|---|---|---|---|---|
| 1 | –3.0 ± 0.2 | –3.1 ± 0.3 | 0.50 ± 0.05 | 0.40 ± 0.03 | 16.8 ± 0.6 |
| 21 | –4.2 ± 0.5 | –4 ± 3 | 0.63 ± 0.09 | 0.4 ± 0.2 | 18 ± 1 |
| 55 | –2.0 ± 0.3 | –2.4 ± 0.3 | 0.27 ± 0.04 | 0.26 ± 0.02 | 13.9 ± 0.9 |
| 60 | –2.7 ± 0.2 | –4.7 ± 0.2 | 0.25 ± 0.02 | 0.30 ± 0.01 | 17 ± 1 |
| 87 | –3.7 ± 0.3 | –6.0 ± 0.6 | 0.38 ± 0.03 | 0.41 ± 0.03 | 17.8 ± 0.4 |
| 116 | –4.0 ± 0.1 | –5 ± 1 | 0.57 ± 0.02 | 0.44 ± 0.07 | 17.2 ± 0.2 |
| 143 | –4.4 ± 0.6 | –3 ± 1 | 1.0 ± 0.1 | 0.6 ± 0.1 | 18 ± 1 |
| 178 | –3.8 ± 0.6 | –1.6 ± 0.4 | 0.8 ± 0.1 | 0.36 ± 0.04 | 17 ± 1 |