Figure 4.

Cotransplantation of ILC2s reduces pro-inflammatory donor T-cell numbers in the GI tract. Donor T cells were evaluated in the GI tract 12 days’ posttransplant by using green fluorescent protein–positive splenic T cells alone (BM + T cells) or green fluorescent protein–positive T cells and B10.BR ILC2s (BM, T cells + B10.BR ILC2s). (A) Frequency of total LP lymphocytes. (B) Total number of donor CD4⁺ and CD8⁺ T cells in the colonic lamina propria lymphocytes. (C) Flow cytometry plots of intracellular cytokine expression from colon. Percentage (D) and total number (E) of IFN-γ producing donor CD4⁺ and CD8⁺ T cells and the number of donor CD4⁺ T cells producing IL-17A in the LP. These data represent 2 independent experiments (n = 5), Student’s t test with Welch’s correction; *P < .05, **P < .01, ***P < .001.